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Titre du document / Document title

Antigenotoxic, antimutagenic and ROS scavenging activities of a Rhoeo discolor ethanolic crude extract

Auteur(s) / Author(s)

GONZALEZ-AVILA M. (1) ; ARRIAGA-ALBA M. (2) ; DE LA GARZA M. (1) ; HERNANDEZPRETELIN M. Del (3) ; DOMINGUEZ-ORTIZ M. A. (4) ; FATTEL-FAZENDA S. (1) ; VILLA-TREVINO S. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados del IPN, Apartado postal 14-740, México D.F. 07000, MEXIQUE
(2) Laboratorio de Investigación en Microbiología, Directión de Investigación y Enseñanza, Hospital Juárez de México, Av. Instituto Politécnico Nacional 5160, México D.F. 07760, MEXIQUE
(3) Facultad de Medicina, Universidad Veracruzana, Atenas y Managua s/n Minatitlán Ver., 96780, MEXIQUE
(4) Instituto de Ciencias Básicas, Universidad Veracruzana, Km 3.5 carretera federal Jalapa-Las Trancas, Jalapa Ver., 96520, MEXIQUE

Résumé / Abstract

Rhoeo discolor is a legendary plant used for treatment of superficial mycoses in Mexican traditional medicine. Despite its extended use, it is not known whether it has side-effects. An ethanolic crude extract from Rhoeo discolor was prepared, its mutagenic capacity was investigated by the Ames test, and its genotoxic activity in primary liver cell cultures using the unscheduled DNA synthesis assay. This extract was not mutagenic when tested with Salmonella typhimurium strains TA97, TA98 and TA100, and it did not elicit unscheduled DNA synthesis in hepatocyte cultures. In addition, we explored the antimutagenic and antigenotoxic activities of the extract and its ROS scavenger behaviour. Our results show that Rhoeo extract is antimutagenic for S. typhimurium strain TA102 pretreated with ROS-generating mutagen norfloxacin in the Ames test, and protects liver cell cultures against diethylnitrosamine induction of unscheduled DNA synthesis even at 1.9 ng per dish, which was the lowest dose tested. A free radical scavenging test was used in order to explore the antioxidant capacity of Rhoeo extract, as compared with three commercial well-known antioxidants quercetin, ascorbic acid and tocopherol. Rhoeo extract showed less radical scavenging effect than quercetin, but similar to that of α-tocopherol and more than ascorbic acid. It is important to note that this extract was neither mutagenic in S. typhimurium nor genotoxic in liver cell culture, even at concentrations as high as four- and 166-fold of those needed for maximal antimutagenic or chemoprotective activities, respectively.

Revue / Journal Title

Toxicology in vitro    ISSN  0887-2333   CODEN TIVIEQ 

Source / Source

2003, vol. 17, no1, pp. 77-83 [7 page(s) (article)] (39 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Oxford, ROYAUME-UNI  (1987) (Revue)

Mots-clés anglais / English Keywords

Bacteria

;

Enterobacteriaceae

;

America

;

Central America

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Commelinaceae

;

S9 mix

;

Salmonella typhimurium

;

Ames test

;

Mutagenicity testing

;

DPPH

;

Free radical

;

Radical scavenger

;

In vitro

;

Antioxidant

;

Antimutagen

;

Anticarcinogen

;

Biological activity

;

Mexico

;

Folk medicine

;

Pharmacognosy

;

Medicinal plant

;

Plant origin

;

Extract

;

Mots-clés français / French Keywords

Bactérie

;

Enterobacteriaceae

;

Amérique

;

Amérique Centrale

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Commelinaceae

;

Fraction S9

;

Salmonella typhimurium

;

Test Ames

;

Test mutagénicité

;

Hydrazyle(diphényl picryl)

;

Radical libre

;

Intercepteur radical

;

In vitro

;

Antioxydant

;

Antimutagène

;

Anticarcinogène

;

Activité biologique

;

Mexique

;

Médecine traditionnelle

;

Pharmacognosie

;

Plante médicinale

;

Origine végétale

;

Extrait

;

Mots-clés espagnols / Spanish Keywords

Bacteria

;

Enterobacteriaceae

;

America

;

America central

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Commelinaceae

;

Fracción S9

;

Salmonella typhimurium

;

Prueba Ames

;

Prueba mutagenicidad

;

Radical libre

;

Interceptor radical

;

In vitro

;

Antioxidante

;

Antimutágeno

;

Anticarcinógeno

;

Actividad biológica

;

México

;

Medicina tradicional

;

Farmacognosia

;

Planta medicinal

;

Origen vegetal

;

Extracto

;

Mots-clés d'auteur / Author Keywords

Antimutagenic

;

Antigenotoxic

;

Rhoeo discolor extract

;

Antioxidant

;

Chemoprotection

;

Localisation / Location

INIST-CNRS, Cote INIST : 21203, 35400010751130.0090

Nº notice refdoc (ud4) : 14486953



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