RefDoc
Haut

Faire une nouvelle recherche
Make a new search
Lancer la recherche


Titre du document / Document title

Evidence for a direct antitumor mechanism of action of Bovine lactoferricin

Auteur(s) / Author(s)

ELIASSEN Liv Tone (1) ; BERGE Gerd (1) ; SVEINBJØRNSSON Baldur (2) ; SVENDSEN John S. (3) ; VORLAND Lars H. (4) ; REKDAL Øystein (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Biochemistry, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, 9037 Tromsø, NORVEGE
(2) Department of Experimental Pathology, Institute of Medical Biology, Faculty of Medicine, University of Tromsø, 9037 Tromsø, NORVEGE
(3) Department of Chemistry, Faculty of Science, University of Tromsø, 9037 Tromsø, NORVEGE
(4) Department of Medical Microbiology, University Hospital, 9038 Tromsø, NORVEGE

Résumé / Abstract

Background: Bovine lactoferrin (LFB) and its pepsin-generated peptide lactoferricin (LfcinB) possess antitumor activities. The mechanism underlying the antitumor activities of LfcinB in vivo has not been elucidated. In this study the antitumor activities exerted by LFB, LfcinB and murine lactoferricin (LfcinM) on murine tumor cell lines and experimental tumors were investigated. Materials and Methods: The protein and peptides were tested against Meth A fibrosarcoma, B16F10 melanoma and C26 colon carcinoma cells in vitro and their derived tumors in vivo, exploring the mechanisms of antitumor activity by way of histological and scanning electron microscopical studies. Results: LfcinB exerted significant cytotoxic activity against the three tumor cell lines in vitro and significantly reduced the size of solid Meth A tumors. Scanning electron micrographs revealed tumor cell membrane disruption and eventually cell lysis, while extensive hemorrhagic necrosis was apparent in tumor sections one day after LfcinB treatment. No species-specific antitumor effect of LfcinM was observed. Conclusion: Our study demonstrated that LfcinB elicits an antitumor effect mediated through a direct mechanism of action not observed with LFB or LfcinM.

Revue / Journal Title

Anticancer research    ISSN  0250-7005 

Source / Source

2002, vol. 22, no5, pp. 2703-2710 [8 page(s) (article)] (37 ref.)

Langue / Language

Anglais

Editeur / Publisher

International Institute of Anticancer Research, Attiki, GRECE  (1980) (Revue)

Mots-clés anglais / English Keywords

Ferroprotein

;

Colonic disease

;

Intestinal disease

;

Digestive diseases

;

Malignant tumor

;

Vertebrata

;

Mammalia

;

Rodentia

;

Sarcoma

;

Animal

;

Mouse

;

Mechanism of action

;

Treatment

;

Chemotherapy

;

In vivo

;

In vitro

;

Antineoplastic agent

;

Lactoferrin

;

Biological activity

;

Melanoma

;

Fibrosarcoma

;

Established cell line

;

Colon

;

Carcinoma

;

Mots-clés français / French Keywords

Ferroprotéine

;

Côlon pathologie

;

Intestin pathologie

;

Appareil digestif pathologie

;

Tumeur maligne

;

Vertebrata

;

Mammalia

;

Rodentia

;

Lignée C26

;

Lignée B16F10

;

Lignée Meth A

;

Sarcome

;

Animal

;

Souris

;

Mécanisme action

;

Traitement

;

Chimiothérapie

;

In vivo

;

In vitro

;

Anticancéreux

;

Lactoferrine

;

Activité biologique

;

Mélanome

;

Fibrosarcome

;

Lignée cellulaire établie

;

Côlon

;

Carcinome

;

Mots-clés espagnols / Spanish Keywords

Ferroproteina

;

Colón patología

;

Intestino patología

;

Aparato digestivo patología

;

Tumor maligno

;

Vertebrata

;

Mammalia

;

Rodentia

;

Sarcoma

;

Animal

;

Ratón

;

Mecanismo acción

;

Tratamiento

;

Quimioterapia

;

In vivo

;

In vitro

;

Anticanceroso

;

Lactoferrina

;

Actividad biológica

;

Melanoma

;

Fibrosarcoma

;

Línea celular establecida

;

Colón

;

Carcinoma

;

Mots-clés d'auteur / Author Keywords

Lactoferricin

;

antitumor activity

;

Localisation / Location

INIST-CNRS, Cote INIST : 19426, 35400010714237.0260

Nº notice refdoc (ud4) : 14397614



Faire une nouvelle recherche
Make a new search
Lancer la recherche
Bas