Titre du document / Document title

Effects of oxymatrine on the serum levels of T helper cell 1 and 2 cytokines and the expression of the S gene in hepatitis B virus S gene transgenic mice: A study on the anti-hepatitis B virus mechanism of oxymatrine

Auteur(s) / Author(s)

YUHONG DONG ⁽¹⁾ ; HONGLI XI ⁽¹⁾ ; YANYAN YU ⁽¹⁾ ; QINHUAN WANG ⁽¹⁾ ; KAN JIANG ⁽²⁾ ; LIUZHE LI ⁽³⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Infectious Disease, Peking University First Hospital, Beijing, CHINE
⁽²⁾ Department of Immunology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, CHINE
⁽³⁾ Department of Parasitology, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, CHINE

Résumé / Abstract

Background: Oxymatrine has been shown to have a remarkable inhibitory activity to hepatitis B virus (HBV) infection with a hepatitis B virus e antigen (HBeAg) serum conversion rate of approximately 45%. In order to explore the anti-HBV mechanism of oxymatrine, the effects of oxymatrine on serum levels of T helper (h) 1 cytokines (interferon (IFN)-y and interleukin (IL)-2) and Th2 cytokines (IL-4 and IL-10), and the expression of S gene in HBV S gene transgenic mice were studied. Methods: Each transgenic mouse was either injected with oxymatrine or saline intraperitoneally once a day for 30 days. Serum levels of IFN-y, IL-2, IL-4 and IL-10 were quantitated and compared to the data before the treatment. The expression of HBV S gene in transgenic mice was analyzed at the DNA, mRNA and protein levels. Results: The serum levels of IFN-y in transgenic mice before or after oxymatrine treatment were 3.108±3.172 and 11.059±6.971 pg/mL, respectively. In contrast, serum levels before and after oxymatrine treatment for IL-4 were 29.045±13.235 and 13.024±9.002 pg/mL. respectively (P<0.001). The serum levels of IL-2 in the control (saline injection) and oxymatrine-treated mice were 1.070±0.447 and 5.537±2.887 pg/mL, respectively (P<0.0001); and that of IL-10 were 97.226±73.306 and 33.607±23.154pg/mL, respectively (P<0.01). No significant differences were observed in the expression of HBV S gene in the transgenic mice at the DNA, mRNA and protein levels before or after oxymatrine treatment. Conclusions: The fact thatThl cytokines are increased while Th2 cytokines are decreased suggests that oxymatrine treatment triggers the change of immune response to hepatitis B infection in transgenic mice, which leads to improved HBV inhibitory activities. The study can help us better understand the mechanisms of the anti-HBV drug, oxymatrine, and how it has potential as an application in clinical chronic hepatitis B treatment.

Revue / Journal Title

Journal of gastroenterology and hepatology   ISSN 0815-9319 

Source / Source

2002, vol. 17, n^o12, pp. 1299-1306 [8 page(s) (article)] (28 ref.)

Langue / Language

Anglais

Editeur / Publisher

Wiley-Blackwell, Richmond, AUSTRALIE  (1986) (Revue)

Mots-clés anglais / English Keywords

Immunology ; Hepatic disease ; Digestive diseases ; Asia ; Vertebrata ; Mammalia ; Rodentia ; Infection ; Viral disease ; China ; Mouse ; Transgenic animal ; Experimental study ; Cytokine ; Biosynthesis ; Helper cell ; Immune response ; Medicinal plant ; Alkaloid ; Chemotherapy ; Biological activity ; Viral hepatitis B ;

Mots-clés français / French Keywords

Immunologie ; Foie pathologie ; Appareil digestif pathologie ; Asie ; Vertebrata ; Mammalia ; Rodentia ; Infection ; Virose ; Oxymatrine ; Chine ; Souris ; Animal transgénique ; Etude expérimentale ; Cytokine ; Biosynthèse ; Cellule helper ; Réponse immune ; Plante médicinale ; Alcaloïde ; Chimiothérapie ; Activité biologique ; Hépatite virale B ;

Mots-clés espagnols / Spanish Keywords

Inmunología ; Hígado patología ; Aparato digestivo patología ; Asia ; Vertebrata ; Mammalia ; Rodentia ; Infección ; Virosis ; China ; Ratón ; Animal transgénico ; Estudio experimental ; Citoquina ; Biosíntesis ; Célula auxiliar ; Respuesta inmune ; Planta medicinal ; Alcaloide ; Quimioterapia ; Actividad biológica ; Hepatitis vírica B ;

Mots-clés d'auteur / Author Keywords

antiviral drug ; hepatitis B virus S gene ; immune response ; oxymatrine ; T helper cell 1 and 2 cytokine ; transgenic mouse ;

Localisation / Location

INIST-CNRS, Cote INIST : 21223, 35400010566587.0100