Titre du document / Document title

Production of recombinant endostatin from stably transformed Trichoplusia ni BTI Tn 5B1-4 cells

Auteur(s) / Author(s)

JONG MIN LEE ⁽¹⁾ ; KYUNG HWA CHANG ⁽¹⁾ ; JONG HWA PARK ⁽¹⁾ ; YOUN HYUNG LEE ⁽²⁾ ; IN SIK CHUNG ^{(1 2)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Genetic Engineering, Kyung Hee University, Suwon 449-701, COREE, REPUBLIQUE DE
⁽²⁾ Plant Metabolism Research Center, Kyung Hee University, Suwon 449-701, COREE, REPUBLIQUE DE

Résumé / Abstract

The recombinant plasmids harboring a heterologous gene coding mouse endostatin were transfected and expressed stably in Trichoplusia ni BTI Tn 5B1-4 (Tn 5B1-4) cells. Recombinant endostatin expressed in the stably transformed Tn 5B1-4 cells was secreted into the medium. Recombinant endostatin was also purilied to homogeneity using a simple one-step Ni²⁺ affinity fractionation method. Purified recombinant endostatin inhibited endothelial cell proliferation in a dose-dependent manner. The concentration at half-maximum inhibition (ED₅₀) for recombinant endostatin was approximately ().35 μg ml^-1. In a T-flask, the stably transformed Tn 5B1-4 cells produced 14.3 mg recombinant endostatin l^-1 at 6 days of cultivation.

Revue / Journal Title

Biotechnology letters   ISSN 0141-5492   CODEN BILED3 

Source / Source

2001, vol. 23, n^o23, pp. 1931-1936 (13 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1979) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Rodentia ; Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Noctuidae ; Recombinant cell ; Purification ; Recombinant protein ; Mouse ; Endostatin ; Trichoplusia ni ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Rodentia ; Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Noctuidae ; Cellule recombinante ; Purification ; Protéine recombinante ; Souris ; Endostatine ; Trichoplusia ni ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Rodentia ; Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Noctuidae ; Célula recombinante ; Purificación ; Proteína recombinante ; Ratón ; Endostatina ; Trichoplusia ni ;

Localisation / Location

INIST-CNRS, Cote INIST : 18225, 35400010315043.0060