Titre du document / Document title

Aldose reductase mRNA expression is associated with rapid development of diabetic microangiopathy in japanese Type 2 diabetic (T2DM) patients

Auteur(s) / Author(s)

SHIMIZU H. ⁽¹⁾ ; OHTANI K.-I. ⁽¹⁾ ; TSUCHIYA T. ⁽¹⁾ ; SATO N. ⁽²⁾ ; TANAKA Y. ⁽¹⁾ ; TAKAHASHI H. ⁽¹⁾ ; UEHARA Y. ⁽¹⁾ ; INUKAI T. ⁽³⁾ ; MORI M. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ First Department of Internal Medicine Gunma University School of Medicine, Gunma, JAPON
⁽²⁾ Department of Endocrinology, Dokkyo University School of Medicine, Tochigi, JAPON
⁽³⁾ Department of Internal Medicine, Dokkyo University School of Medicine, Koshigaya Hospital, Saitama, JAPON

Résumé / Abstract

There appears to be an obvious difference in the susceptibility to diabetic microangiopathy among Type 2 diabetic (T2DM) patients. We examined the hypothesis that aldose reductase (AR) messenger ribonucleic acid (mRNA) expression may be enhanced in diabetic patients susceptible to diabetic microangiopathy by measuring AR mRNA expression in the polymorphonuclear leukocytes (PMNs) obtained from T2DM patients. The AR mRNA and β-actin mRNA expressions were measured by reverse transcription-polymerase chain reaction method. The relative AR mRNA expression to β-actin mRNA expression significantly increased in the PMNs of T2DM patients, compared to normal healthy controls. The relative AR mRNA expression tended to increase with an increase of glycosylated haemoglobin A_1c (HbA_1c) levels. The patients who showed both progressive diabetic retinopathy and diabetic neuropathy within 10 years after the diagnosis of T2DM (Group I) showed significantly 1.6 times higher AR mRNA expressions than those who have not shown both complications over 10 years after diagnosis (Group II), in spite of no obvious difference in glycaemic control between both groups. The present data indicate that the AR mRNA expression increased in the PMNs of T2DM patients and there is an obvious difference in the PMNs AR mRNA expression between T2DM patients susceptible and relatively resistant to the development of diabetic microangiopathy.

Revue / Journal Title

Diabetes, nutrition & metabolism   ISSN 0394-3402 

Source / Source

Congrès
International Symposium on Amino Acid/Protein Metabolism in Health and Disease: State of the art at the turn of the Millennium N^o5
2000, vol. 13, n^o 2, pp. 92-112 (32 ref.), pp. 75-79

Langue / Language

Anglais

Editeur / Publisher

Kurtis, Milano, ITALIE  (1988-2004) (Revue)

Mots-clés anglais / English Keywords

Non insulin dependent diabetes ; Complication ; Microangiopathy ; Aldehyde reductase ; Messenger RNA ; Genetic determinism ; Prognosis ; Human ; Oxidoreductases ; Enzyme ; Endocrinopathy ; Vascular disease ; Cardiovascular disease ;

Mots-clés français / French Keywords

Diabète non insulinodépendant ; Complication ; Microangiopathie ; Aldehyde reductase ; RNA messager ; Déterminisme génétique ; Pronostic ; Homme ; Oxidoreductases ; Enzyme ; Endocrinopathie ; Vaisseau sanguin pathologie ; Appareil circulatoire pathologie ;

Mots-clés espagnols / Spanish Keywords

Diabetes no insulinodependiente ; Complicación ; Microangiopatía ; Aldehyde reductase ; RNA mensajero ; Determinismo genético ; Pronóstico ; Hombre ; Oxidoreductases ; Enzima ; Endocrinopatía ; Vaso sanguíneo patología ; Aparato circulatorio patología ;

Localisation / Location

INIST-CNRS, Cote INIST : 21709, 35400008895063.0030