Titre du document / Document title

Cytopathogenicity of Acanthamoeba isolates on rat glial C6 cell line

Auteur(s) / Author(s)

LAGMAY J. P. ⁽¹⁾ ; MATIAS R. R. ⁽²⁾ ; NATIVIDAD F. F. ⁽²⁾ ; ENRIQUEZ G. L. ⁽³⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Mindanao State University, Marawi City, PO Box 5512, Iligan City 9200, PHILIPPINES
⁽²⁾ St Luke's Medical Center, Cathedral Heights, 279 E Rodriguez Sr Blvd, Quezon City 1102, PHILIPPINES
⁽³⁾ Natural Sciences Research Institute, UP Diliman, Quezon City, PHILIPPINES

Résumé / Abstract

The pathogenicity of Acanthamoeba isolates from keratitis patients (the Hamburg isolate from Germany, H-1 and a Philippine isolate, IB-1-7) as well as an environmental isolate, W4 was assayed in vitro using rat glial C6 cell line. Results indicate that both live amebae and cell-free supernatants from H-1 and IB-1-7 clones produced cytopathic effects (CPE) on rat glial C6 cells in a dose-and-time-dependent fashion A dose of 10⁵ cells/ml induced death and moderate areas of destruction of individual cells after 48 hours of incubation. Results of both free zone capillary electrophoresis and sodium dodecyl sulphate polyacryla mide gel electrophoresis suggest the release of amebic products to the culture medium that could at least partially explain the observed cytopathogenicity after 48 hours. Furthermore, results of SDS-PAGE indicate differences between the secretions of the isolates, with bands produced by the two ocular isolates that were not seen with the environmental isolates. That the secretions can produce a cytopathic effect (CPE) has been shown by the cytotoxicity assays using protein concentrations of the secretory products. Protein concen tration of 0.30 μg/μl of culture supernatants from H-1 and IB-1-7 clones produced similar effects on the cell monolayers after 2 hours of incubation. This concentration caused the highest % cell death as measured by both trypan blue exclusion (TBE) and 3-(4,5-dimethylthiazol 2-yl) 2,5-diphenyl-tetrazolium bromide (MTT) assays. In contrast, using W4 clone, corresponding concentrations of both trophozoites and culture supematant did not cause significant cell death and cellular disintegration.

Revue / Journal Title

Southeast Asian journal of tropical medicine and public health   ISSN 0125-1562   CODEN SJTMAK 

Source / Source

1999, vol. 30, n^o4, pp. 670-677 (16 ref.)

Langue / Language

Anglais

Editeur / Publisher

Southeast Asian Ministers of Education Organization, Regional Tropical Medicine and Public Health Network, Bangkok, THAILANDE  (1970) (Revue)

Mots-clés anglais / English Keywords

Keratitis ; Acanthamoeba ; Protozoal disease ; Clinical isolate ; Cytotoxicity ; Cell line ; Rat ; Animal ; Lobosea ; Protozoa ; Parasitosis ; Infection ; Rodentia ; Mammalia ; Vertebrata ; Eye disease ; Keratopathy ;

Mots-clés français / French Keywords

Kératite ; Acanthamoeba ; Protozoose ; Isolat clinique ; Cytotoxicité ; Lignée cellulaire ; Rat ; Animal ; Lobosea ; Protozoa ; Parasitose ; Infection ; Rodentia ; Mammalia ; Vertebrata ; Oeil pathologie ; Kératopathie ;

Mots-clés espagnols / Spanish Keywords

Queratitis ; Acanthamoeba ; Protozoosis ; Aislado clinico ; Citotoxicidad ; Línea celular ; Rata ; Animal ; Lobosea ; Protozoa ; Parasitosis ; Infección ; Rodentia ; Mammalia ; Vertebrata ; Ojo patología ; Queratopatía ;

Localisation / Location

INIST-CNRS, Cote INIST : 19778, 35400008731292.0110