Titre du document / Document title

Calorie restriction increases insulin-stimulated tyrosine phosphorylation of insulin receptor and insulin receptor substrate-1 in rat skeletal muscle

Auteur(s) / Author(s)

DEAN D. J. ⁽¹⁾ ; CARTEE G. D. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Biodynamics Laboratory, Department of Kinesiology and Department of Nutritional Sciences, University of Wisconsin, Madison, WI, ETATS-UNIS

Résumé / Abstract

A moderate reduction in calorie intake (calorie restriction, CR) improves insulin-stimulated glucose transport in skeletal muscle Therefore, we studied muscle insulin signalling in ad libitum (AL) and CR (-60% AL intake for 20 days) fed rats, which received a control injection (sterile water) or an insulin injection (30 U kg^-1 body weight). In control (not insulin-treated) rats, there was no detectable tyrosine phosphorylation of insulin receptor (IR), regardless of diet; no diet effect on tyrosine phosphorylation of insulin receptor substrate-1 (IRS1) or IRS1-associated phosphatidylinositol 3-kinase (P13K) protein and 21% higher IRS1-associated P13K activity in AL vs. CR. In insulin-treated rats, tyrosine-phosphorylated IR was 79% higher for CR vs. AL; tyrosine-phosphorylated IRS1 was 109% higher for CR vs. AL; IRS1-associated P13K protein and IRS1-associated P13K activity were unaffected by diet. Calorie restriction amplifies early insulin signalling steps without changing IRS1-associated P13K, suggesting enhanced glucose transport is mediated by altering: IRS1-P13K localization, P13K associated with proteins other than IRS1 or post-P13K events.

Revue / Journal Title

Acta physiologica scandinavica   ISSN 0001-6772   CODEN APSCAX 

Source / Source

2000, vol. 169, n^o2, pp. 133-139 (27 ref.)

Langue / Language

Anglais

Editeur / Publisher

Blackwell Science, Oxford, ROYAUME-UNI  (1940-2005) (Revue)

Mots-clés anglais / English Keywords

Calorie ; Restricted diet ; Insulin ; Hormonal receptor ; Gastrocnemius muscle ; Phosphorylation ; Tyrosine ; Signal transduction ; Kinase ; Rat ; Animal ; Transferases ; Enzyme ; Feeding ; Pancreatic hormone ; Striated muscle ; Rodentia ; Mammalia ; Vertebrata ;

Mots-clés français / French Keywords

Calorie ; Régime alimentaire restrictif ; Insuline ; Récepteur hormonal ; Muscle gastrocnémien ; Phosphorylation ; Tyrosine ; Transduction signal ; Kinase ; Rat ; Animal ; Phosphatidylinositol-3-kinase ; Transferases ; Enzyme ; Alimentation ; Hormone pancréatique ; Muscle strié ; Rodentia ; Mammalia ; Vertebrata ;

Mots-clés espagnols / Spanish Keywords

Caloría ; Régimen alimenticio restrictivo ; Insulina ; Receptor hormonal ; Músculo gastrocnémio ; Fosforilación ; Tirosina ; Transducción señal ; Kinase ; Rata ; Animal ; Transferases ; Enzima ; Alimentación ; Hormona pancreática ; Músculo estriado ; Rodentia ; Mammalia ; Vertebrata ;

Localisation / Location

INIST-CNRS, Cote INIST : 907 A, 35400008856073.0060