Titre du document / Document title

Rhizobium specificity of Trifolium ambiguum M. Bieb × T. repens L. hybrid clovers

Auteur(s) / Author(s)

LOWTHER W. L. ⁽¹⁾ ; PRYOR H. N. ⁽¹⁾ ; LITTLEJOHN R. P. ⁽¹⁾ ; HUSSAIN S. W. ⁽²⁾ ; WILLLIAMS W. M. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ AgResearch, Invermay Agricultural Centre, Private Bag 50034, Mosgiel, NOUVELLE-ZELANDE
⁽²⁾ AgResearch, Grasslands, Private Bag 11008, Palmerston North, NOUVELLE-ZELANDE

Résumé / Abstract

Growth of full-sib families of an F₁ interspecific hybrid between Trifolium ambiguum M.Bieb and T. repens L, and two generations of backcross hybrids (BC₁F₂ and BC₂F₁) with T. repens as the recurrent parent, were compared to their parental species. Plants were grown in a N-free medium and inoculated with Rhizobium leguminosarum biovar trifolii rhizobia effective on T. ambiguum or T. repens. Hybridisation produced progeny that nodulated with rhizobia from either T. ambiguum or T. repens, but plant growth varied. Mean weights of T. repens and hybrids, particularly F₁ and BC₁F₂, were higher when inoculated with a mixture of strains isolated from field grown T. repens than with the New Zealand inoculant strain for T. repens. When inoculated with the mix of rhizobia from T. repens, mean weights were 242, 189, 132, 125, and 100 mg/plant for T. repens, BC₂F₁, BC₁F₂, F₁ and T. ambiguum, respectively. However, although the mean weight of BC₂F₁ lines was significantly less than T. repens, there was considerable variation in individual full-sib families indicating the potential to select within BC₂F₁ hybrids for high plant growth/symbiotic nitrogen fixation. The weight of T. ambiguum plants inoculated with the New Zealand inoculant strain for hexaploid T. ambiguum was similar to T. repens inoculated with the mix of rhizobia from T. repens (253 and 242 mg/plant, respectively). Mean fresh weights of F₁ hybrid plants were similar when inoculated with rhizobia for T. ambiguum or T. repens (125 and 130 mg/plant, respectively). However, weight of T. repens, BC₁F₂ and BC₂F₁ hybrids inoculated with rhizobia for T. ambiguum were all less than 90 mg/plant. There was a significant relationship between plant fresh weight and ethylene production. The results indicate that measuring weights of inoculated plants growing in N-free media is a rapid initial method of screening a range of plant germplasm for plant growth/symbiotic nitrogen fixation rates.

Revue / Journal Title

Euphytica   ISSN 0014-2336   CODEN EUPHAA 

Source / Source

2002, vol. 127, n^o3, pp. 309-315 [7 page(s) (article)] (20 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1952) (Revue)

Mots-clés anglais / English Keywords

Symbionte ; Fodder crop ; Plant microorganism relation ; Bacteria ; Rhizobiaceae ; Spermatophyta ; Angiospermae ; Dicotyledones ; Leguminosae ; Rhizobium leguminosarum bv. trifolii ; Trifolium repens ; Nitrogen fixation ; Nodulation ; Interspecific hybridization ;

Mots-clés français / French Keywords

Symbionte ; Plante fourragère ; Relation microorganisme végétal ; Bactérie ; Rhizobiaceae ; Spermatophyta ; Angiospermae ; Dicotyledones ; Leguminosae ; Trifolium ambiguum ; Rhizobium leguminosarum bv. trifolii ; Trifolium repens ; Fixation azote ; Nodulation ; Hybridation interspécifique ;

Mots-clés espagnols / Spanish Keywords

Simbionte ; Planta forrajera ; Relación microorganismo vegetal ; Bacteria ; Rhizobiaceae ; Spermatophyta ; Angiospermae ; Dicotyledones ; Leguminosae ; Rhizobium leguminosarum bv. trifolii ; Trifolium repens ; Fijación nitrogeno ; Nodulación ; Hibridación interespecífica ;

Localisation / Location

INIST-CNRS, Cote INIST : 8245, 35400010651694.0020