Titre du document / Document title
Little evidence for developmental plasticity of adult hematopoietic stem cells
Auteur(s) / Author(s)
WAGERS Amy J. ;
SHERWOOD Richard I. ;
CHRISTENSEN Julie L. ;
WEISSMAN Irving L. ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
Department of Pathology and Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, ETATS-UNIS
Résumé / Abstract
To rigorously test the in vivo cell fate specificity of bone marrow (BM) hematopoietic stem cells (HSCs), we generated chimeric animals by transplantation of a single green fluorescent protein (GFP)-marked HSC into lethally irradiated nontransgenic recipients. Single HSCs robustly reconstituted peripheral blood leukocytes in these animals, but did not contribute appreciably to nonhematopoietic tissues, including brain, kidney, gut, liver, and muscle. Similarly, in GFP
+:GFP
- parabiotic mice, we found substantial chimerism of hematopoietic but not nonhematopoietic cells. These data indicate that transdifferentiation of circulating HSCs and/or their progeny is an extremely rare event, if it occurs at all.
Revue / Journal Title
Science
ISSN 0036-8075
CODEN SCIEAS
Source / Source
2002, vol. 297, n
o5590, pp. 2256-2259 [4 page(s) (article)]
Langue / Language
Anglais
Editeur / Publisher
American Association for the Advancement of Science, Washington, DC, ETATS-UNIS
(1880)
(Revue)
Mots-clés anglais / English Keywords
Vertebrata ;
Mammalia ;
Rodentia ;
Cell lineage ;
Homograft ;
Graft ;
Chimera ;
Mouse ;
Animal ;
Hematopoiesis ;
Plasticity ;
Hematopoietic cell ;
Developmental biology ;
Transdifferentiation ;
Stem cell ;
Cell differentiation ;
Mots-clés français / French Keywords
Vertebrata ;
Mammalia ;
Rodentia ;
Lignage cellulaire ;
Homogreffe ;
Greffe ;
Chimère ;
Souris ;
Animal ;
Hématopoïèse ;
Plasticité ;
Cellule hématopoïétique ;
Biologie développement ;
Transdifférenciation ;
Cellule souche ;
Cytodifférenciation ;
Mots-clés espagnols / Spanish Keywords
Vertebrata ;
Mammalia ;
Rodentia ;
Líneación celular ;
Homoinjerto ;
Injerto ;
Quimera ;
Ratón ;
Animal ;
Hematopoyesis ;
Plasticidad ;
Célula hematopoyética ;
Biología desarrollo ;
Transdiferenciación ;
Célula primitiva ;
Diferenciación celular ;
Localisation / Location
INIST-CNRS, Cote INIST : 6040, 35400010513423.0230
Nº notice refdoc (ud4) : 13962301
