Titre du document / Document title

Little evidence for developmental plasticity of adult hematopoietic stem cells

Auteur(s) / Author(s)

WAGERS Amy J. ; SHERWOOD Richard I. ; CHRISTENSEN Julie L. ; WEISSMAN Irving L. ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

Department of Pathology and Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, ETATS-UNIS

Résumé / Abstract

To rigorously test the in vivo cell fate specificity of bone marrow (BM) hematopoietic stem cells (HSCs), we generated chimeric animals by transplantation of a single green fluorescent protein (GFP)-marked HSC into lethally irradiated nontransgenic recipients. Single HSCs robustly reconstituted peripheral blood leukocytes in these animals, but did not contribute appreciably to nonhematopoietic tissues, including brain, kidney, gut, liver, and muscle. Similarly, in GFP⁺:GFP^- parabiotic mice, we found substantial chimerism of hematopoietic but not nonhematopoietic cells. These data indicate that transdifferentiation of circulating HSCs and/or their progeny is an extremely rare event, if it occurs at all.

Revue / Journal Title

Science   ISSN 0036-8075   CODEN SCIEAS 

Source / Source

2002, vol. 297, n^o5590, pp. 2256-2259 [4 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

American Association for the Advancement of Science, Washington, DC, ETATS-UNIS  (1880) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Rodentia ; Cell lineage ; Homograft ; Graft ; Chimera ; Mouse ; Animal ; Hematopoiesis ; Plasticity ; Hematopoietic cell ; Developmental biology ; Transdifferentiation ; Stem cell ; Cell differentiation ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Rodentia ; Lignage cellulaire ; Homogreffe ; Greffe ; Chimère ; Souris ; Animal ; Hématopoïèse ; Plasticité ; Cellule hématopoïétique ; Biologie développement ; Transdifférenciation ; Cellule souche ; Cytodifférenciation ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Rodentia ; Líneación celular ; Homoinjerto ; Injerto ; Quimera ; Ratón ; Animal ; Hematopoyesis ; Plasticidad ; Célula hematopoyética ; Biología desarrollo ; Transdiferenciación ; Célula primitiva ; Diferenciación celular ;

Localisation / Location

INIST-CNRS, Cote INIST : 6040, 35400010513423.0230