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Titre du document / Document title

Concept of sequential analysis of free and conjugated phytosterols in different plant matrices

Auteur(s) / Author(s)

BREINHÖLDER Patrick (1) ; MOSCA Livia (1) ; LINDNER Wolfgang (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Institute of Analytical Chemistry, University of Vienna, Währingerstraβe 38, 1090 Vienna, AUTRICHE

Résumé / Abstract

A unique concept and method for the determination of the total plant sterol content as sum of free sterols (FS), steryl esters (SE), steryl glycosides (SG) and acylated steryl glycosides (ASG) in different plant materials (pumpkin seeds. lecithins) and phytopharmaceuticals derived thereof, was developed. For this purpose, a multidimensional sample clean-up protocol based on efficient solid-phase extraction materials was elaborated and the SG were isolated employing a novel phenyl boronic acid modified silica gel material. Along this line also a set of steryl glucosides was synthesised and employed as internal standard and for calibration in the course of quantitative analysis. Final quantification of SG was carried out with reversed-phase HPLC in combination with evaporative light scattering detection (ELSD); the ASG were determined after conversion to SG by mild alkaline hydrolysis. In order to determine the total plant sterol profile the sum of FS and SE was additionally analysed from the unsaponifiable lipid fraction by GC-FID. The yields obtained from recovery tests for the determination of SG using soya lecithin as matrix to which 2, 20 and 40 mg/g of cholesterol-β-D-glucoside was added were 99.10, 98.07 and 90.00%, and the RSDs were 4.11, 2.62 and 4.50%, respectively. Application related to the qualitative and quantitative analysis of total phytosterol profiles in different plant matrices and extracts demonstrate the validity of the method.

Revue / Journal Title

Journal of chromatography. B, Biomedical sciences and applications    ISSN  1387-2273 

Source / Source

2002, vol. 777, no 1-2 (333 p.)  [Document : 16 p.] (25 ref.), pp. 67-82 [16 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1997-2001) (Revue)

Mots-clés anglais / English Keywords

Qualitative analysis

;

Sequential analysis

;

HPLC chromatography

;

Solid phase extraction

;

Multiple system

;

Glycoside

;

Glucoside

;

Gas chromatography

;

Investigation method

;

Quantitative analysis

;

Plant origin

;

Seeds

;

Phytosterol

;

Mots-clés français / French Keywords

Analyse qualitative

;

Analyse séquentielle

;

Chromatographie HPLC

;

Extraction SPE

;

Système multiple

;

Glycoside

;

Glucoside

;

Chromatographie phase gazeuse

;

Méthode étude

;

Analyse quantitative

;

Origine végétale

;

Graine

;

Phytostérol

;

Mots-clés espagnols / Spanish Keywords

Análisis cualitativo

;

Análisis secuencial

;

Cromatografía HPLC

;

Extracción SPE

;

Sistema múltiple

;

Glicósido

;

Glucósido

;

Cromatografía fase gaseosa

;

Método estudio

;

Análisis cuantitativo

;

Origen vegetal

;

Semillas

;

Fitosterol

;

Localisation / Location

INIST-CNRS, Cote INIST : 8577 B, 35400010490382.0050

Nº notice refdoc (ud4) : 13922958



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