RefDoc
Haut

Faire une nouvelle recherche
Make a new search
Lancer la recherche


Titre du document / Document title

Arabidopsis plants transformed with nitrilase 1 or 2 in antisense direction are delayed in clubroot development

Auteur(s) / Author(s)

NEUHAUS K. (1) ; GRSIC-RAUSCH S. (1) ; SAUERTEIG S. (1) ; LUDWIG-MÜLLER J. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Botanisches Institut, Johann Wolfgang Goethe-Universität, Siesmayerstrasse 70, 60054 Frankfurt, ALLEMAGNE

Résumé / Abstract

The infection of Arabidopsis thaliana with the causal agent of the clubroot disease, Plasmodiophora brassicae Wor., leads to tumorous swellings of the root as a result of increased cell division and hypertrophy. Recently, it was shown that nitrilase 1 and 2 were prominently enhanced in clubroots compared to controls. To investigate the role of nitrilase for symptom development, Arabidopsis thaliana plants transformed with nitrilase 1 and 2 in antisense direction (aNIT 1, aNIT2) were infected with P brassicae. The infection rate of aNIT 1 plants was reduced compared to the wild type, whereas the infection rate was unaffected in aNIT2 plants. The size of root galls was reduced in aNIT1 plants throughout club development, whereas aNIT2 plants were only delayed in club development and showed similar club weight as the wild type 42 days after inoculation. A smaller number of fungal structures was observed in aNIT1 and aNIT2 plants and the resting spores were not clustered within large sporangia compared to a typical clubroot infection. The reduced fresh weights correlated with reduced nitrilase mRNA and protein, thus resulting in lower free IAA content in the smaller clubs of the antisense plants.

Revue / Journal Title

Journal of plant physiology    ISSN  0176-1617   CODEN JPPHEY 

Source / Source

2000, vol. 156, no5-6, pp. 756-761 (31 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Munich, ALLEMAGNE  (1984) (Revue)

Mots-clés anglais / English Keywords

Transgenic plant

;

Nitrilase

;

Antisense DNA

;

Host agent relation

;

Infection

;

Concentration

;

Arabidopsis thaliana

;

Plasmodiophora brassicae

;

Hydrolases

;

Enzyme

;

Cruciferae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Mycetozoa

;

Protozoa

;

Experimental plant

;

Plant pathogen

;

Indoleacetic acid

;

Plant growth substance

;

Mots-clés français / French Keywords

Plante transgénique

;

Nitrilase

;

DNA antisens

;

Relation hôte agent

;

Infection

;

Concentration

;

Arabidopsis thaliana

;

Plasmodiophora brassicae

;

Indole-3-acétonitrile

;

Indole-3-acétique acide

;

Hydrolases

;

Enzyme

;

Cruciferae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Mycetozoa

;

Protozoa

;

Plante expérimentale

;

Phytopathogène

;

Indoleacétique acide

;

Substance croissance végétal

;

Mots-clés espagnols / Spanish Keywords

Planta transgénica

;

Nitrilase

;

DNA antisentido

;

Relación huesped agente

;

Infección

;

Concentración

;

Arabidopsis thaliana

;

Plasmodiophora brassicae

;

Hydrolases

;

Enzima

;

Cruciferae

;

Dicotyledones

;

Angiospermae

;

Spermatophyta

;

Mycetozoa

;

Protozoa

;

Planta experimental

;

Fitopatógeno

;

Indoloacetico ácido

;

Substancia crecimiento vegetal

;

Localisation / Location

INIST-CNRS, Cote INIST : 922, 35400008895634.0280

Nº notice refdoc (ud4) : 1391288



Faire une nouvelle recherche
Make a new search
Lancer la recherche
Bas