Titre du document / Document title

Protective effect of Fructus Lycii polysaccharides against time and hyperthermia-induced damage in cultured seminiferous epithelium

Auteur(s) / Author(s)

YANRONG WANG ⁽¹⁾ ; HUI ZHAO ⁽²⁾ ; XINSHENG SHENG ⁽¹⁾ ; GAMBINO Patricia E. ⁽³⁾ ; COSTELLO Brian ⁽³⁾ ; BOJANOWSKI Krzysztof ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Histology and Embryology, Ningxia Medical College, Yinchuan 750004, CHINE
⁽²⁾ Sunny BioDiscovery, Inc., 675 Hegenberger Rd., 2nd Floor, Oakland, CA 94621, ETATS-UNIS
⁽³⁾ The Collaborative Group, Ltd., 50 East Loop Rd., Stony Brook, NY 11790, ETATS-UNIS

Résumé / Abstract

Lycium barbarum L. (Solanaceae) is a Chinese medicinal plant whose fruits (Fructus Lycii) are used by Chinese physicians for treatment of infertility. However, the active ingredients and the mechanism of action underlying Lycium's fertility-facilitating effects remain unknown. Here we report that Fructus Lycii polysaccharides (FLPS) inhibit time- and hyperthermia-induced structural damage in murine seminiferous epithelium, in vitro. Moreover, we found that FLPS delayed apoptosis in this system, both at normothermic and hyperthermic culture conditions. Oxidative stress was reported to be a major cause of structural degradation and apoptosis in hyperthermic testes, and thus the protective effect of FLPS could implicate an antioxidant mechanism of action. To test this hypothesis we assayed the effect of FLPS on ultraviolet light-induced lipid peroxidation, and cytochrome c reduction by free radicals. We found that FLPS is a potent inhibitor of both of these reactions. Together, these results demonstrate the protective effect of FLPS on time- and hyperthermia-induced testicular degeneration in vitro, indicate the potential mechanism of action for this protective effect, and provide a scientific basis for the traditional use of this plant.

Revue / Journal Title

Journal of ethnopharmacology   ISSN 0378-8741   CODEN JOETD7 

Source / Source

2002, vol. 82, n^o2-3, pp. 169-175 (24 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Shannon, IRLANDE  (1978) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Rodentia ; Spermatophyta ; Angiospermae ; Dicotyledones ; Solanaceae ; Spermatogenesis ; Spermatozoa ; Asia ; Mouse ; Animal ; Peroxidation ; Antioxidant ; Oxidative stress ; Cytoprotector ; Cell death ; Apoptosis ; In vitro ; Fertility ; Seminal vesicle ; Seminiferous tubule ; Mechanism of action ; Fruit ; Chinese medicine ; China ; Folk medicine ; Induced hyperthermia ; Polysaccharide ; Male genital system ; Pharmacognosy ; Testicle ; Oxygen ; Medicinal plant ; Free radical ; Lipids ; Plant origin ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Rodentia ; Spermatophyta ; Angiospermae ; Dicotyledones ; Solanaceae ; Spermatogenèse ; Spermatozoïde ; Asie ; Lycium barbarum ; Souris ; Animal ; Peroxydation ; Antioxydant ; Stress oxydatif ; Cytoprotecteur ; Mort cellulaire ; Apoptose ; In vitro ; Fertilité ; Vésicule séminale ; Canalicule séminifère ; Mécanisme action ; Fruit ; Médecine chinoise ; Chine ; Médecine traditionnelle ; Hyperthermie provoquée ; Polyoside ; Appareil génital mâle ; Pharmacognosie ; Testicule ; Oxygène ; Plante médicinale ; Radical libre ; Lipide ; Origine végétale ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Rodentia ; Spermatophyta ; Angiospermae ; Dicotyledones ; Solanaceae ; Espermatogénesis ; Espermatozoide ; Asia ; Ratón ; Animal ; Peroxidación ; Antioxidante ; Estrés oxidativo ; Citoprotector ; Muerte celular ; Apoptosis ; In vitro ; Fertilidad ; Vesícula seminal ; Túbulo seminífero ; Mecanismo acción ; Fruto ; Medicina china ; China ; Medicina tradicional ; Hipertermia provocada ; Poliósido ; Aparato genital macho ; Farmacognosia ; Testículo ; Oxígeno ; Planta medicinal ; Radical libre ; Lípido ; Origen vegetal ;

Localisation / Location

INIST-CNRS, Cote INIST : 18028, 35400010473537.0150