Titre du document / Document title

Novel growth factors involved in the pathogenesis of proliferative vitreoretinopathy

Auteur(s) / Author(s)

HINTON D. R. ^{(1 2)} ; HE S. ⁽²⁾ ; JIN M. L. ⁽²⁾ ; BARRON E. ⁽³⁾ ; RYAN S. J. ^{(1 3)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, CA, ETATS-UNIS
⁽²⁾ Department of Pathology, Keck School of Medicine of the University of Southern California, Los Angeles, CA, ETATS-UNIS
⁽³⁾ Doheny Eye Institute, Los Angeles, CA, ETATS-UNIS

Résumé / Abstract

Aims To determine whether hepatocyte growth factor (HGF) and connective tissue growth factor (CTGF) are expressed in human specimens of proliferative vitreoretinopathy (PVR) and to propose a model of PVR pathogenesis based upon the known activities of these growth factors. Methods Immunohistochemical methods (ABC Elite) were used to demonstrate the presence of HGF and CTGF in cryostat sections of five human PVR membranes. Results In each of the five PVR membranes, stromal cells were immunohistochemically positive for both HGF and CTGF. Based upon this information and the known actions of these growth factors, a model of PVR pathogenesis was developed. In this model, injury of the retina induces an inflammatory response that upregulates HGF expression inducing the formation of multilayered groups of migratory retinal pigment epithelial cells (RPE). These RPE, present in a provisional extracellular matrix, come in contact with vitreous containing TGF-β. The TGF-β is activated, upregulating expression of CTGF. Under the influence of TGF-β and CTGF, RPE become myofibroblastic and fibrosis ensues. Retinal traction induces further detachment continuing the cycle of retinal injury. Conclusions HGF and CTGF are expressed in PVR membranes and may play important roles in the pathogenesis of PVR. The expression and function of these growth factors should be critically examined in human PVR specimens, in in vitro cultures of RPE, and in animal models of PVR.

Revue / Journal Title

Eye   ISSN 0950-222X   CODEN EYEEEC 

Source / Source

Congrès
Cambridge Ophthalmological Symposium, Cambridge , ROYAUME-UNI (09/2001)
2002, vol. 16, n^o4, pp. 422-428 (62 ref.)

Langue / Language

Anglais

Editeur / Publisher

Nature Publishing Group, Basingstoke, ROYAUME-UNI  (1987) (Revue)

Mots-clés anglais / English Keywords

Pathology ; Vitreous body disease ; Retinopathy ; Eye disease ; Human ; Immunohistochemistry ; Growth factor ; Connective tissue ; Hepatocyte growth factor ; Pathogenesis ; Proliferative vitreoretinopathy ;

Mots-clés français / French Keywords

Anatomopathologie ; Corps vitré pathologie ; Rétinopathie ; Oeil pathologie ; Homme ; Immunohistochimie ; Facteur croissance ; Tissu conjonctif ; Facteur croissance hépatocyte ; Pathogénie ; Vitréorétinopathie proliférante ;

Mots-clés espagnols / Spanish Keywords

Anatomía patológica ; Cuerpo vidrioso patología ; Retinopatía ; Ojo patología ; Hombre ; Inmunohistoquímica ; Factor crecimiento ; Tejido conjuntivo ; Factor crecimiento hepatocito ; Patogenia ; Vitreorretinopatía proliferante ;

Localisation / Location

INIST-CNRS, Cote INIST : 21076, 35400010443522.0110