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Titre du document / Document title

Use of the SMITEST PSA card to identify the presence of prostate-specific antigen in semen and male urine

Auteur(s) / Author(s)

SATO Itaru (1 2) ; SAGI Morihisa (1) ; ISHIWARI Atsuya (2) ; NISHIJIMA Hironori (1) ; ITO Emi (1) ; MUKAI Toshiji (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Legal Medicine, St. Marianna University School of Medicine, 2-16-1 Sugao, Mivamae-ku, Kawasaki, Kanagawa 216-8511, JAPON
(2) Scientific Crime Laboratory, Kanagawa Prefectural Police Headquarters, Yokohama, JAPON

Résumé / Abstract

To determine whether the prostate-specific antigen (PSA) could be identified in semen using the SMITEST PSA immunochromatographie membrane test card, we examined semen and other body fluids, including urine. Although PSA activity was detected in semen with high sensitivity using the SMITEST PSA card, it was also detected in adult male urine. However, the lower detectable limit in the urine was 1000-fold lower than that in semen. The concentration of PSA in adult male urine was found to be 800 ng/ml using the card. PSA activity usually can he detected in urine of individuals over 14 years old and it has been detected in urine from children as young as I I years old. Therefore, the appearance of PSA in urine may occur anytime between the age of 12 and 14 years. To determine the stability of PSA activity in urine, dried samples of urine on filter paper were kept at room temperature for up to 3 years. Although the immunoreactive line showing PSA activity became weak after storage, it was still detectable, hut faint, after 3 years. In addition, PSA activity was not detected in male serum or saliva and in the urine from human females, male cats or male dogs using the PSA card. We conclude that the PSA card is useful for identification of PSA in both semen and adult male urine.

Revue / Journal Title

Forensic science international    ISSN  0379-0738   CODEN FSINDR 

Source / Source

2002, vol. 127, no1-2, pp. 71-74 (16 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Kidlington, ROYAUME-UNI  (1978) (Revue)

Mots-clés anglais / English Keywords

Human

;

Rate

;

Age

;

Urine

;

Male

;

Semen

;

Prostate specific antigen

;

Tumoral marker

;

Use

;

Detection

;

Technique

;

Biological fluid

;

Identification

;

Mots-clés français / French Keywords

SMITEST PSA card

;

Homme

;

Taux

;

Age

;

Urine

;

Mâle

;

Sperme

;

Antigène spécifique prostate

;

Marqueur tumoral

;

Utilisation

;

Détection

;

Technique

;

Liquide biologique

;

Identification

;

Mots-clés espagnols / Spanish Keywords

Hombre

;

Tasa

;

Edad

;

Orina

;

Macho

;

Esperma

;

Antigeno específico prostata

;

Marcador tumoral

;

Utilización

;

Detección

;

Técnica

;

Líquido biológico

;

Identificación

;

Localisation / Location

INIST-CNRS, Cote INIST : 15440, 35400010187400.0040

Nº notice refdoc (ud4) : 13760389



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