Titre du document / Document title

AFLP in triticum aestivum L.: patterns of genetic diversity and genome distribution

Auteur(s) / Author(s)

HAZEN Samuel P. ⁽¹⁾ ; LEROY Phillipe ⁽²⁾ ; WARD Richard W. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI 48824, ETATS-UNIS
⁽²⁾ UMR INRA-UBP Amélioration et Santé des Plantes, Site de Crouelle, 63 039 Clermont-Ferrand, FRANCE

Résumé / Abstract

The amplified fragment length polymorphism (AFLP) procedure was applied to a diverse panel of wheat (Triticum aestivum L. em. Thell.) accessions and sixty-nine of the recombinant inbred lines (RILs) from the widely used genetic mapping population derived from the cross of Opata 85 and W7984. Most (76.8%) bands were monomorphic among T. aestivum accessions. The majority of bands monomorphic in T. aestivum also were present in the synthetic wheat parent (W7984). Ten primer pairs generated 153 polymorphic AFLP bands, 140 of which could be assigned to a chromosome location and were relatively evenly distributed on the genetic linkage map. AFLP loci in T. aestivum were distributed throughout the genome; they generally have only one detectable sequence variant; and they exhibit monogenic dominant mendelian inheritance. Frequencies of polymorphic bands in the germplasm sampled are in the range that enables informative cluster analyses as well as map-based diversity and association analysis studies. AFLP bands mapped to individual loci in the Opata 85/W7984 RIL population will frequently be polymorphic in other crosses or germplasm, irrespective of whether the band arises from the T. aestivum parent or the synthetic wheat parent.

Revue / Journal Title

Euphytica   ISSN 0014-2336   CODEN EUPHAA 

Source / Source

2002, vol. 125, n^o1, pp. 89-102 (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1952) (Revue)

Mots-clés anglais / English Keywords

Cereal crop ; Genetic marker ; Spermatophyta ; Angiospermae ; Monocotyledones ; Gramineae ; Genetic distance ; Germplasm ; Phylogenetic tree ; Triticum aestivum ; Genetic improvement ; Polymorphism ; Genetic mapping ; Linkage group ; Intraspecific comparison ; Genome ; Genetic variability ; Genetic diversity ; Amplified fragment length polymorphism marker ;

Mots-clés français / French Keywords

Plante céréalière ; Marqueur génétique ; Spermatophyta ; Angiospermae ; Monocotyledones ; Gramineae ; Distance génétique ; Germoplasme ; Arbre phylogénétique ; Triticum aestivum ; Amélioration génétique ; Polymorphisme ; Carte génétique ; Groupe liaison ; Comparaison intraspécifique ; Génome ; Variabilité génétique ; Diversité génétique ; Marqueur AFLP ;

Mots-clés espagnols / Spanish Keywords

Planta cerealista ; Marcador genético ; Spermatophyta ; Angiospermae ; Monocotyledones ; Gramineae ; Distancia genética ; Germoplasma ; Arbol filogenético ; Triticum aestivum ; Enriquecimiento genético ; Polimorfismo ; Mapa genético ; Grupo ligamiento ; Comparación intraespecífica ; Genoma ; Variabilidad genética ; Diversidad genética ; Marcador AFLP ;

Localisation / Location

INIST-CNRS, Cote INIST : 8245, 35400010174051.0110