Titre du document / Document title

LC determination and pharmacokinetics of meloxicam

Auteur(s) / Author(s)

DASANDI Bhavesh ⁽¹⁾ ; SHIVAPRAKASH ⁽¹⁾ ; SAROJ H. ⁽¹⁾ ; BHAT K. M. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Synchron Research Services Pvt. Ltd., Sarkhej Gandhinagar Highway, Bodakdev, Ahmedabad 380 054, INDE
⁽²⁾ K.B. Institute of Pharmaceutical Education and Research, GH-6, Sector-23, Gandhinagar 382 023, INDE

Résumé / Abstract

A simple and rapid HPLC assay method for the estimation of meloxicam in plasma was developed. The method totally eliminated the solvent extraction procedure. The plasma proteins were precipitated using perchloric acid (70%) and acetonitrile mixture (1:1 v/v) and the supernatant was directly injected to the HPLC system. The separation was achieved on a Lichrospher C₁₈ 5μ (125 x 4.0 mm) analytical column with a mobile phase of sodium acetate buffer (pH 3.3, 170 mmol):acetonitrile (62:38 v/v) mixture. Detection was by UV detector at 355 nm. The retention time observed for meloxicam and piroxicam (internal standard) were at 6.0 and 4.0 min, respectively. The response was linear over a range of 50 1500 ng ml^-1 in human plasma. The method was simple, specific, precise and accurate. The method was also used for the bioequivalence study of meloxicam formulation in healthy, human, Indian, male volunteers.

Revue / Journal Title

Journal of pharmaceutical and biomedical analysis   ISSN 0731-7085   CODEN JPBADA 

Source / Source

2002, vol. 28, n^o5, pp. 999-1004 (10 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1983) (Revue)

Mots-clés anglais / English Keywords

Enzyme ; Oxidoreductases ; Enzyme inhibitor ; Prostaglandin-endoperoxide synthase ; Oxicam derivatives ; Ultraviolet detector ; HPLC chromatography ; Single dose ; Oral administration ; Human ; Biological fluid ; Blood plasma ; Pharmacokinetics ; Analysis method ; Quantitative analysis ; Non steroidal antiinflammatory agent ; Meloxicam ;

Mots-clés français / French Keywords

Enzyme ; Oxidoreductases ; Benzothiazine dérivé ; Inhibiteur enzyme ; Prostaglandin-endoperoxide synthase ; Oxicam dérivé ; Détecteur UV ; Chromatographie HPLC ; Dose unique ; Voie orale ; Homme ; Liquide biologique ; Plasma sanguin ; Pharmacocinétique ; Méthode analyse ; Analyse quantitative ; Antiinflammatoire non stéroïde ; Méloxicam ;

Mots-clés espagnols / Spanish Keywords

Enzima ; Oxidoreductases ; Inhibidor enzima ; Prostaglandin-endoperoxide synthase ; Oxicam derivado ; Detector UV ; Cromatografía HPLC ; Dosis única ; Vía oral ; Hombre ; Líquido biológico ; Plasma sanguíneo ; Farmacocinética ; Método análisis ; Análisis cuantitativo ; Antiinflamatorio no esteroide ; Meloxicam ;

Localisation / Location

INIST-CNRS, Cote INIST : 19962, 35400010153360.0230