Titre du document / Document title

Effect of Xalatan® and Cosopt® on the vascular tone of quiescent isolated pig ciliary arteries

Auteur(s) / Author(s)

EL SHERBINI Mustafa ⁽¹⁾ ; GEKKIEVA Margarita ⁽¹⁾ ; FLAMMER Josef ⁽¹⁾ ; HAEFLIGER Ivan O. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Laboratory of Ocular Pharmacology and Physiology, University Eye Clinic Basel, SUISSE

Résumé / Abstract

Background: To investigate whether dilutions of the commercially available Xalatan® or Cosopt® induce vasoconstrictions in isolated quiescent porcine ciliary arteries. Material and methods: Contractions measured with a myograph system (isometric force measurement) were expressed in percent of 100 mM potassium chloride(KCl)-induced contraction (mean ± standard error). In preliminary experiments, vessels were contracted with 100 mM KCl after previous exposures to different dilutions of XaIatan® (1/250 to 1/50) or Cosopt® (1/500 to 1/5). In a further series of experiments, vessels were exposed in a cumulative manner to different dilutions of Xalatan® or Cosopt® (1/50 000 to 1/ 166). Results: Contractions induced by 100 mM KCl were not significantly affected by previous exposure to Xalatan® or Cosopt® at dilutions higher than 1/166 and 1/50, respectively. However, at the lower dilutions tested, 100 mM KCl-induced contractions were significantly (p < 0.001) and markedly inhibited (1/50 Xalatan®: 22.1 ± 2.8%; 1/5 Cosopt®: 24.8 ± 4.6%). In quiescent vessels, diluted (1/50000 to 1/166) preparations of Xalatan® induced, in a dilution-dependent manner, statistically significant (p < 0.05) but, however very small (3.2 ± 1.0%) contractions in comparison to Cosopt® (- 0.6 ± 0.2%). Conclusions: Although on the basis of these in vitro experiments no clinical interpretation should be made, at very high dilutions that did not affect KCl-induced contractions, Xalatan®, but not Cosopt®, had a very small vasoconstrictive effect in isolated quiescent porcine ciliary arteries. At low dilutions, responses to KCl were inhibited, possibly in relation to an unclear effect of the excipients of Xalatan® and Cosopt®.

Revue / Journal Title

Klinische Monatsblätter für Augenheilkunde   ISSN 0023-2165   CODEN KMAUAI 

Source / Source

2002, vol. 219, n^o4, pp. 273-276 (16 ref.)

Langue / Language

Anglais
Revue : Allemand

Editeur / Publisher

Thieme, Stuttgart, ALLEMAGNE  (1963) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Ungulata ; Artiodactyla ; Sulfonamide ; Prostaglandin derivatives ; Ocular ; Biological activity ; Vasoconstriction ; Blood flow ; Posterior ciliary artery ; Anterior ciliary artery ; Animal ; Pig ; Timolol ; In vitro ; Beta blocking agent ; Dorzolamide ; Hemodynamics ; Antiglaucomatous agent ; Latanoprost ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Ungulata ; Artiodactyla ; Sulfonamide ; Prostaglandine dérivé ; Oculaire ; Activité biologique ; Vasoconstriction ; Débit sanguin ; Artère ciliaire postérieure ; Artère ciliaire antérieure ; Animal ; Porc ; Timolol ; In vitro ; Bloquant β-adrénergique ; Dorzolamide ; Hémodynamique ; Antiglaucomateux ; Latanoprost ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Ungulata ; Artiodactyla ; Sulfonamida ; Prostaglandina derivado ; Ocular ; Actividad biológica ; Vasoconstricción ; Flujo sanguíneo ; Arteria ciliar posterior ; Arteria ciliar anterior ; Animal ; Cerdo ; Timolol ; In vitro ; Bloqueador β-adrenérgico ; Dorzolamida ; Hemodinámica ; Antiglaucomatoso ; Latanoprost ;

Localisation / Location

INIST-CNRS, Cote INIST : 3850 A, 35400010149459.0190