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Titre du document / Document title

Regulation of rat ileal NHE3 by 1,25(OH)2-Vitamin D3

Auteur(s) / Author(s)

GILL R. (1) ; NAZIR T. M. (1) ; WALI R. (2) ; SITRIN T. M. (2) ; BRASITUS T. A. (2) ; RAMASWAMY K. (1) ; DUDEJA P. K. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Medicine, University of Illinois at Chicago and VAMC Chicago (Westside), Chicago, Illinois 60612, ETATS-UNIS
(2) University of Chicago, Chicago, Illinois 60637, ETATS-UNIS

Résumé / Abstract

We have previously demonstrated a modulation of Na+/H+ exchange (NHE) activity by vitamin D3 in the rat ileum and Caco-2 cells. However, the molecular mechanism(s) of action of vitamin D3 on NHE are still not understood. The current studies were undertaken to understand the regulation of individual NHE isoforms on mRNA levels in two distinct models of vitamin D3 deficiency. Acute D3 deficiency was induced secondary to streptozotocin-induced diabetes mellitus, while chronic D3 deficiency was induced by feeding a D3-deficient diet in an environment devoid of fluorescent light. Vitamin D3 deficiency in both models increased the initial rates of rat ileal brush-border membrane (BBM) Na+/H+ exchange by 2.5-fold compared to D-repleted controls. In parallel to the increased exchanger activity, NHE3 mRNA abundance was increased about twofold in both acute and chronic D deficiency compared to control. There was no change in NHE1 or NHE2 abundance in vitamin D3-deficient rat ileum. These findings indicate that vitamin D3 regulates Na+/H+ exchange activity in rat ileum by influencing the mRNA levels of NHE3, the predominant luminal membrane isoform involved in vectorial Na+ transport.

Revue / Journal Title

Digestive diseases and sciences    ISSN  0163-2116   CODEN DDSCDJ 

Source / Source

2002, vol. 47, no5, pp. 1169-1174 (26 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (1979) (Revue)

Mots-clés anglais / English Keywords

Vitamin

;

Electrolyte

;

Digestive diseases

;

Vertebrata

;

Mammalia

;

Rodentia

;

Rat

;

Animal

;

Colecalciferol

;

Deficiency

;

Regulation(control)

;

Hydrogen ion

;

Sodium

;

Ion exchange

;

Ileum

;

Mots-clés français / French Keywords

Vitamine

;

Electrolyte

;

Appareil digestif pathologie

;

Vertebrata

;

Mammalia

;

Rodentia

;

Rat

;

Animal

;

Colécalciférol

;

Déficit

;

Régulation

;

Hydrogène ion

;

Sodium

;

Echange ion

;

Iléon

;

Mots-clés espagnols / Spanish Keywords

Vitamina

;

Electrolito

;

Aparato digestivo patología

;

Vertebrata

;

Mammalia

;

Rodentia

;

Rata

;

Animal

;

Colecalciferol

;

Déficiencia

;

Regulación

;

Hidrógeno ión

;

Sodio

;

Cambio iónico

;

Ileon

;

Localisation / Location

INIST-CNRS, Cote INIST : 5060, 35400010818764.0320

Nº notice refdoc (ud4) : 13674335



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