Titre du document / Document title

Ocular toxicity of intravitreal tacrolimus

Auteur(s) / Author(s)

PASSOS Elke ⁽¹⁾ ; GENAIDY Mahmoud M. ⁽¹⁾ ; PEYMAN Gholam A. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Ophthalmology, Tulane University Health Sciences Center, New Orleans, Louisiana, ETATS-UNIS

Résumé / Abstract

■ BACKGROUND AND OBJECTIVE: To investigate the ocular toxicity of intravitreally administered tacrolimus, a drug with potent immunosuppressive activity. ■ METHODS: To evaluate toxicity, tacrolimus was injected into the midvitreous cavity of 20 eyes of New Zealand pigmented rabbits at concentrations of 10, 50, 100, 250, 500, and 1000 μg. Control eyes received balanced salt solution. Eyes receiving 1000 μg were given injections of 0.2 mL solution; all others, including controls, received 0.1 mL. Rabbits were examined before the injections by slit-lamp biomicroscopy, indirect ophthalmoscopy, and an electroretinography test (ERG) was performed. The animals were followed up to 14 days postinjection by clinical examination and ERG. The animals were killed and the eyes were enucleated and processed for light microscopy. was seen in the eyes receiving 10 or 50 μg of tacrolimus. One out of 4 eyes that received 100 μg of the drug developed a vitreous reaction. All eyes treated with 250 μg or more developed vitreous reaction. One eye injected with 1000 μg of the drug developed occlusion of the temporal retinal vessels. Electroretinography showed decreasing b-wave amplitude with both dark- and light-adapted stimulus in the 500 and 1000 μg groups, and it was normal in the other groups. Histopathologic sections showed mild disorganization of the retina only at the 500 and 1000 μg dosage. ■ CONCLUSIONS: Doses of 10 and 50 μg of tacrolimus are nontoxic to rabbit eyes. Only transient vitreous opacities were observed in the groups that received 100 and 250 μg. Intravitreal doses of 500 and 1000 μg of tacrolimus proved to be toxic to the retina.

Revue / Journal Title

Ophthalmic surgery and lasers   ISSN 1082-3069   CODEN OSLAF2 

Source / Source

2002, vol. 33, n^o2, pp. 140-144 (13 ref.)

Langue / Language

Anglais

Editeur / Publisher

Slack, Thorofare, NJ, ETATS-UNIS  (1995-2000) (Revue)

Mots-clés anglais / English Keywords

Electrodiagnosis ; Vertebrata ; Mammalia ; Lagomorpha ; Macrocycle ; Lactone ; Rabbit ; Animal ; Retina ; Electroretinography ; Eye ; Vitreous body ; Injection ; Toxicity ; Immunosuppressive agent ; Tacrolimus ;

Mots-clés français / French Keywords

Electrodiagnostic ; Vertebrata ; Mammalia ; Lagomorpha ; Macrocycle ; Lactone ; Lapin ; Animal ; Rétine ; Electrorétinographie ; Oeil ; Corps vitré ; Injection ; Toxicité ; Immunodépresseur ; Tacrolimus ;

Mots-clés espagnols / Spanish Keywords

Electrodiagnóstico ; Vertebrata ; Mammalia ; Lagomorpha ; Macrociclo ; Lactona ; Conejo ; Animal ; Retina ; Electrorretinografía ; Ojo ; Cuerpo vidrioso ; Inyección ; Toxicidad ; Inmunodepresor ; Tacrolimús ;

Localisation / Location

INIST-CNRS, Cote INIST : 14946, 35400010795236.0080