Titre du document / Document title

Detection of tetQ and ermF antibiotic resistance genes in Prevotella and Porphyromonas isolates from clinical specimens and resident microbiota of humans

Auteur(s) / Author(s)

ARZESE A. R. ⁽¹⁾ ; TOMASETIG L. ⁽¹⁾ ; BOTTA G. A. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Institute of Microbiology, Faculty of Medicine, University of Udine, 33100 Udine, ITALIE

Résumé / Abstract

Gram-negative anaerobes belonging to the genera Fusobacterium, Prevotella and Porphyromonas were investigated for the presence of tetQ and ermF, which have been shown to be spread by conjugal elements. One hundred isolates from either sites of infection or various body sites in healthy subjects were studied. PCR was used to detect tetQ, and DNA-DNA hybridization studies on EcoRI chromosomal digests were undertaken to detect the presence of tetQ and ermF. Antibiotic sensitivity assays were performed on selected isolates to detect tetracycline, erythromycin and penicillin resistance. Twenty Fusobacterium isolates lacked tetQ, and were tetracycline sensitive. Twenty per cent of Prevotella spp. isolates both from clinical specimens and from healthy subjects were found to possess tetQ. Of 20 Porphyromonas isolates tested, one (Porphyromonas levii) from a case of bacterial vaginosis was shown to possess tetQ in the chromosome. The presence of tetQ was always associated with tetracycline resistance. Four isolates of Prevotella melaninogenica and one isolate of Prevotella were ermF-positive, although expression of erythromycin resistance was not consistently associated with detection of this gene. Antibiotic resistance phenotypes of Prevotella isolates were shown to be related to specific chromosomal restriction patterns by hybridization studies: tetracycline resistance and tetracycline/erythromycin resistance are conferred by Bacteroides tetracycline-resistant ERL elements, whereas the tetracycline/penicillin resistance phenotype could be due to spread of elements identified in Prevotella only. Tetracycline/erythromycin-resistant and tetracycline/erythromycin/penicillin-resistant P. melaninogenica isolates were found In this study. It appeared that the presence of tetQ and ermF in Bacteroides and Prevotella contributed to the persistence of antibiotic resistance isolates within the host and to potential spread to other organisms through conjugal elements.

Revue / Journal Title

Journal of antimicrobial chemotherapy   ISSN 0305-7453   CODEN JACHDX 

Source / Source

2000, vol. 45, n^o5, pp. 577-582 (20 ref.)

Langue / Language

Anglais

Editeur / Publisher

Oxford University Press, Oxford, ROYAUME-UNI  (1975) (Revue)

Mots-clés anglais / English Keywords

Tetracycline ; Erythromycin ; Penicillin ; Antibiotic ; Antibacterial agent ; Resistance ; Prevotella ; Porphyromonas ; Gene expression ; Polymerase chain reaction ; Genetics ; Molecular hybridization ; DNA ; Bacteroides ; Tetracycline derivatives ; Macrolide ; Penicillin derivatives ; β-Lactams ; Bacteroidaceae ; Bacteria ;

Mots-clés français / French Keywords

Tétracycline ; Erythromycine ; Pénicilline ; Antibiotique ; Antibactérien ; Résistance ; Prevotella ; Porphyromonas ; Expression génique ; Réaction chaîne polymérase ; Génétique ; Hybridation moléculaire ; DNA ; Bacteroides ; Tétracycline dérivé ; Macrolide ; Pénicilline dérivé ; β-Lactamines ; Bacteroidaceae ; Bactérie ;

Mots-clés espagnols / Spanish Keywords

Tetraciclina ; Eritromicina ; Antibiótico ; Antibacteriano ; Resistencia ; Prevotella ; Porphyromonas ; Expresión genética ; Reacción cadena polimerasa ; Genética ; Hibridación molecular ; DNA ; Bacteroides ; Tetraciclina derivado ; Macrólido ; Penicilinas ; β-Lactams ; Bacteroidaceae ; Bacteria ;

Localisation / Location

INIST-CNRS, Cote INIST : 17084, 35400008836703.0040