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Titre du document / Document title

Structural identification of a bacterial quorum-sensing signal containing boron

Auteur(s) / Author(s)

XIN CHEN (1) ; SCHAUDER Stephan (1) ; POTIER Noelle (2) ; VAN DORSSELAER Alain (2) ; PELCZER Istvan (3) ; BASSLER Bonnie L. (1) ; HUGHSON Frederick M. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544-1014, ETATS-UNIS
(2) Laboratoire de Spectrometrie de Masse Bio-Organique, Ecole de Chimie, Polymeres et Materiaux, 25 Rue Becquerel, 67087 Strasbourg, FRANCE
(3) Department of Chemistry, Princeton University, Princeton, New Jersey 08544-1014, ETATS-UNIS

Résumé / Abstract

Cell-cell communication in bacteria is accomplished through the exchange of extracellular signalling molecules called autoinducers. This process, termed quorum sensing, allows bacterial populations to coordinate gene expression. Community cooperation probably enhances the effectiveness of processes such as bioluminescence, virulence factor expression, antibiotic production and biofilm development1-4. Unlike other autoinducers, which are specific to a particular species of bacteria, a recently discovered autoinducer (AI-2)5 is produced by a large number of bacterial species. AI-2 has been proposed to serve as a universal' signal for inter-species communication1,2,6,7. The chemical identity of AI-2 has, however, proved elusive. Here we present the crystal structure of an AI-2 sensor protein, LuxP, in a complex with autoinducer. The bound ligand is a furanosyl borate diester that bears no resemblance to previously characterized autoinducers. Our findings suggest that addition of naturally occurring borate to an AI-2 precursor generates active AI-2. Furthermore, they indicate a potential biological role for boron, an element required by a number of organisms but for unknown reasons.

Revue / Journal Title

Nature    ISSN  0028-0836   CODEN NATUAS 

Source / Source

2002, vol. 415, no6871, pp. 545-549 (31 ref.)

Langue / Language

Anglais

Editeur / Publisher

Nature Publishing Group, London, ROYAUME-UNI  (1869) (Revue)

Mots-clés anglais / English Keywords

Bacteria

;

Vibrionaceae

;

Autoinducer

;

Quorum sensing

;

Activation

;

Biological activity

;

Borates

;

Boron

;

Crystalline structure

;

Structural analysis

;

Characterization

;

Protein

;

Cell cell interaction

;

Microorganism interrelationships

;

Vibrio harveyi

;

Mots-clés français / French Keywords

Bactérie

;

Vibrionaceae

;

Autoinducteur

;

Quorum sensing

;

Protéine LuxP

;

Protéine AI2

;

Activation

;

Activité biologique

;

Borate

;

Bore

;

Structure cristalline

;

Analyse structurale

;

Caractérisation

;

Protéine

;

Interaction cellulaire

;

Interaction microorganisme

;

Vibrio harveyi

;

Mots-clés espagnols / Spanish Keywords

Bacteria

;

Vibrionaceae

;

Activación

;

Actividad biológica

;

Borato

;

Boro

;

Estructura cristalina

;

Análisis estructural

;

Caracterización

;

Proteína

;

Interacción celular

;

Interacción microorganismo

;

Vibrio harveyi

;

Localisation / Location

INIST-CNRS, Cote INIST : 142, 35400010263128.0240

Nº notice refdoc (ud4) : 13513721



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