Titre du document / Document title

Hormone sensitive lipase expression and adipose tissue metabolism show gender difference in obese subjects after weight loss

Auteur(s) / Author(s)

KOLEHMAINEN M. ⁽¹⁾ ; VIDAL H. ⁽²⁾ ; OHISALO J. J. ⁽³⁾ ; PIRINEN E. ⁽⁴⁾ ; ALHAVA E. ⁽⁴⁾ ; UUSITUPA M. I. J. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Clinical Nutrition, University of Kuopio and Kuopio University Hospital, Kuopio, FINLANDE
⁽²⁾ INSERM U449, Faculté de Médecine R Laennec, Lyon, FRANCE
⁽³⁾ Institute of Biomedicine, University of Helsinki, FINLANDE
⁽⁴⁾ Department of Surgery, Kuopio University Hospital, FINLANDE

Résumé / Abstract

OBJECTIVE: The effect of weight reduction on hormone sensitive lipase (HSL) and lipoprotein lipase (LPL) gene expression and their relationship with adipose tissue metabolism were studied in massively obese men and women. SUBJECTS: Seventeen obese subjects (eight men, nine women) participated in the study (age 44±2 y, weight 145 ± 8 kg, fat 40 ± 2% of body mass, mean ± s.e.m.), who were going through a gastric-banding operation for weight reduction. MEASUREMENTS: HSL and LPL mRNA expressions were analyzed using the reverse transcription competitive polymerase chain reaction. Subcutaneous fat lipolysis was measured in vivo by microdialysis and in vitro in isolated subcutaneous abdominal adipocytes. Measurements were done before and after 1 y of weight reduction. RESULTS: Significant reductions in weight (for men - 20.3 ± 2.5%, for women -18.3 ± 2.1% (mean ± s.e.m.) and fat mass (for men -27.6 ± 7.9%, for women -21.8 ± 3.9%) were observed in both genders. In women HSL mRNA expression decreased by 31% (P = 0.008) and LPL expression increased slightly, but nonsignificantly (42%, P = 0.110). These changes were not observed in men. In men, inhibition of lipolysis with α₂-adrenergic and adenosine agonist was improved (P=0.001) in isolated adipocytes. CONCLUSIONS: This study uncovers new differences between genders in adipocyte metabolism along with weight reduction. In women, the observed changes in HSL and LPL gene expression suggest that deposition of lipids into adipose tissue might be favored after weight reduction. In men, the results indicate improved responsiveness to inhibition in adipose tissue metabolism along with weight reduction.

Revue / Journal Title

International journal of obesity   ISSN 0307-0565   CODEN IJOBDP 

Source / Source

2002, vol. 26, n^o1, pp. 6-16 (52 ref.)

Langue / Language

Anglais

Editeur / Publisher

Nature Publishing, Basingstoke, ROYAUME-UNI  (1977) (Revue)

Mots-clés anglais / English Keywords

Nutrition disorder ; Enzyme ; Hydrolases ; Esterases ; Carboxylic ester hydrolases ; Weight ; Weight loss ; Human ; Nutritional status ; Sex ; Metabolism ; Triacylglycerol lipase ; Sensitivity ; Hormone ; Fat mass ; Female ; Lipoprotein lipase ; Gene expression ; Adenosine ; Stomach ; Adipose tissue ; Polymerase chain reaction ; Obesity ;

Mots-clés français / French Keywords

Trouble nutrition ; Enzyme ; Hydrolases ; Esterases ; Carboxylic ester hydrolases ; Poids ; Perte poids ; Homme ; Etat nutritionnel ; Sexe ; Métabolisme ; Triacylglycerol lipase ; Sensibilité ; Hormone ; Masse grasse ; Femelle ; Lipoprotein lipase ; Expression génique ; Adénosine ; Estomac ; Tissu adipeux ; Réaction chaîne polymérase ; Obésité ;

Mots-clés espagnols / Spanish Keywords

Trastorno nutricíon ; Enzima ; Hydrolases ; Esterases ; Carboxylic ester hydrolases ; Peso ; Adelgazamiento ; Hombre ; Estado nutricional ; Sexo ; Metabolismo ; Triacylglycerol lipase ; Sensibilidad ; Hormona ; Masa grasa ; Hembra ; Lipoprotein lipase ; Expresión genética ; Adenosina ; Estómago ; Tejido adiposo ; Reacción cadena polimerasa ; Obesidad ;

Localisation / Location

INIST-CNRS, Cote INIST : 18243, 35400010250752.0020