RefDoc
Haut

Faire une nouvelle recherche
Make a new search
Lancer la recherche


Titre du document / Document title

Bioaccumulation of chlorpyrifos through an experimental food chain: Study of protein HSP70 as biomarker of sublethal stress in fish

Auteur(s) / Author(s)

VARO I. (1) ; SERRANO R. (2) ; PITARCH E. (2) ; AMAT F. (1) ; LOPEZ F. J. (2) ; NAVARRO J. C. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Instituto de Acuicultura de Torre de la Sal, C.S.I.C., 12595 Ribera de Cabanes, Castellón, ESPAGNE
(2) Analytical Chemistry, Department of Experimental Sciences, University Jaume 1, P.O. Box 224, 12080 Castellón, ESPAGNE

Résumé / Abstract

The accumulation and transfer of the organophosphorus pesticide chlorpyrifos has been studied in an experimental aquatic two-level food chain using two species of the crustacean Artemia (A. franciscana and A. parthenogenetica) and the small fish Aphanius iberus. Artemia adults contaminated by exposure to the pesticide in water were used as live prey for Aphanius, the next trophic level. During the experimental bioaccumulation phase, fish were fed chlorpyrifos-contaminated Artemia pools with concentrations between 6.5 and 14.5 ng/g fresh weight for 32 days. Both concentrations accumulated in fish, and biomagnification factor (BMF) values showed a continuous decrease during the bioaccumulation phase, probably due to the physicochemical characteristics of the organophosphorus pesticide, to the biotransformation ability of fish and to the progressive adaptation of fish metabolism to toxic exposure. The first day that fish were fed uncontaminated preys, the pesticide accumulated via food was rapidly eliminated and was not detected. The effect of chlorpyrifos exposure through the food chain on stress protein (HSP70) synthesis was measured as a general biochemical response of stress in the fish (A. iberus). The levels of HSP70 were significantly higher in fish fed on contaminated Artemia than in the control fish fed on uncontaminated Artemia. Results showed that the HSP70 induction in fish could be associated to exposure of chlorpyrifos via food.

Revue / Journal Title

Archives of environmental contamination and toxicology    ISSN  0090-4341   CODEN AECTCV 

Source / Source

2002, vol. 42, no2, pp. 229-235 (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (1973) (Revue)

Mots-clés anglais / English Keywords

Vertebrata

;

Pisces

;

Invertebrata

;

Arthropoda

;

Crustacea

;

Branchiopoda

;

Organophosphorus compounds

;

Pesticides

;

Feeding

;

Water pollution

;

Brackish water environment

;

Artemia

;

Chlorpyrifos

;

Insecticide

;

Biological marker

;

Induction

;

Heat shock protein

;

Stress

;

Toxicity

;

Trophic chain

;

Exposure

;

Experimental study

;

Biological accumulation

;

Mots-clés français / French Keywords

Cyprinodontidae

;

Vertebrata

;

Pisces

;

Invertebrata

;

Arthropoda

;

Crustacea

;

Branchiopoda

;

Organophosphoré

;

Pesticide

;

Alimentation

;

Pollution eau

;

Milieu saumâtre

;

Aphanius iberus

;

Artemia

;

Chlorpyrifos

;

Insecticide

;

Marqueur biologique

;

Induction

;

Protéine choc thermique

;

Stress

;

Toxicité

;

Chaîne alimentaire

;

Exposition

;

Etude expérimentale

;

Accumulation biologique

;

Mots-clés espagnols / Spanish Keywords

Vertebrata

;

Pisces

;

Invertebrata

;

Arthropoda

;

Crustacea

;

Branchiopoda

;

Organofosforado

;

Plaguicida

;

Alimentación

;

Contaminación agua

;

Medio salobre

;

Artemia

;

Clorpirifós

;

Insecticida

;

Marcador biológico

;

Inducción

;

Proteína choque térmico

;

Estrés

;

Toxicidad

;

Cadena alimenticia

;

Exposición

;

Estudio experimental

;

Acumulación biológica

;

Localisation / Location

INIST-CNRS, Cote INIST : 15790, 35400010017656.0120

Nº notice refdoc (ud4) : 13480670



Faire une nouvelle recherche
Make a new search
Lancer la recherche
Bas