Titre du document / Document title

Prevention of corneal keratocyte apoptosis after argon fluoride excimer laser irradiation with the free radical scavenger ubiquinone Q10

Auteur(s) / Author(s)

BRANCATO R. ^{(1 2)} ; SCHIAVONE N. ^{(2 3)} ; SIANO S. ^{(2 4)} ; LAPUCCI A. ^{(2 3)} ; PAPUCCI L. ^{(2 3)} ; DONNINI M. ^{(2 3)} ; FORMIGLI L. ^{(2 5)} ; ZECCHI ORLANDINI S. ^{(2 5)} ; CARELLA G. ^{(1 2)} ; CARONES F. ⁽¹⁾ ; CAPACCIOLI S. ^{(2 3)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Ophthalmology and Visual Sciences, San Raffaele Hospital, University of Milano, ITALIE
⁽²⁾ Research Group on Apoptosis applied to Ophthalmology, University Hospital San Raffaele, Milano, ITALIE
⁽³⁾ Department of Experimental Pathology and Oncology, University of Firenze, ITALIE
⁽⁴⁾ Quantum Electronics Institute, National Research Council, Firenze, ITALIE
⁽⁵⁾ Department of Anatomy, Histology and Forensic Medicine, University of Firenze, ITALIE

Résumé / Abstract

PURPOSE. To assess in vitro the potential of the free radical scavenger ubiquinone Q10 in preventing keratocyte apoptosis after argon fluoride (ArF) excimer laser irradiation. METHODS. Cultured rabbit keratocytes were irradiated at very low single-pulse laser fluences. The cumulative effects generated by three total fluence doses between 12 and 45 mJ/cm², representative of single-pulse subablative doses during photorefractive keratectomy (PRK) in humans, were evaluated. We employed the following parameters to compare pretreated (10 μM ubiquinone Q10) and untreated samples: 1) number and morphology of living cells by Trypan blue test and ultramicroscopy, respectively; 2) level of free-radical formation assessed by malonaldehyde quantitation; 3) cellular energy level evaluated by ATP assay. RESULTS. Excimer laser irradiation kills cultured keratocytes by inducing apoptosis. The effect increases with the cumulative fluence dose. In the samples pretreated with ubiquinone Q10 there were significantly fewer cumulative apoptotic events than in the untreated ones. Quantitative analysis of malonaldehyde cellular levels suggested this protective action of ubiquinone Q10 was connected with its ability to scavenge laser-generated free radicals. ATP assay also confirmed that it raised cellular energy levels. CONCLUSIONS. The treatment of corneal keratocytes with relatively low concentrations of ubiquinone Q10 can prevent apoptosis after ArF excimer laser irradiation. If these findings are confirmed on human keratocytes this treatment could be usefully exploited in the PRK surgical procedure. That might lead to a reduction in the occurrence of haze and curvature regression triggered by programmed cell death.

Revue / Journal Title

European Journal of Ophthalmology   ISSN 1120-6721 

Source / Source

2000, vol. 10, n^o1, pp. 32-38 (35 ref.)

Langue / Language

Anglais

Editeur / Publisher

Wichtig, Milano, ITALIE  (1991) (Revue)

Mots-clés anglais / English Keywords

Photorefractive keratectomy ; Excimer ; Laser ; Argon ; Chemoprophylaxis ; Prevention ; Apoptosis ; Keratocyte ; Cornea ; Radical scavenger ; Free radical ; Oxygen ; Ubiquinone ; Cell culture ; Rabbit ; Lagomorpha ; Mammalia ; Vertebrata ; Surgery ;

Mots-clés français / French Keywords

Kératectomie photoréfractive ; Excimère ; Laser ; Argon ; Chimioprophylaxie ; Prévention ; Apoptose ; Kératocyte ; Cornée ; Intercepteur radical ; Radical libre ; Oxygène ; Ubiquinone ; Culture cellulaire ; Lapin ; Lagomorpha ; Mammalia ; Vertebrata ; Chirurgie ;

Mots-clés espagnols / Spanish Keywords

Queratectomía fotorefractiva ; Excímero ; Laser ; Argón ; Quimioprofilaxis ; Prevención ; Apoptosis ; Queratocito ; Córnea ; Interceptor radical ; Radical libre ; Oxígeno ; Ubiquinona ; Cultivo celular ; Conejo ; Lagomorpha ; Mammalia ; Vertebrata ; Cirugía ;

Localisation / Location

INIST-CNRS, Cote INIST : 22516, 35400008642168.0060