Titre du document / Document title

Development and validation of a new high-performance liquid chromatographic estimation method of meloxicam in biological samples

Auteur(s) / Author(s)

VELPANDIAN T. ⁽¹⁾ ; JAISWAL J. ⁽¹⁾ ; BHARDWAJ R. K. ⁽¹⁾ ; GUPTA S. K. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Pharmacokinetic Laboratory, Department of Pharmacology, All India Institute of Medical Sciences, New Delhi-110 029, INDE

Résumé / Abstract

A simple, HPLC method was developed to estimate meloxicam (COX-2 inhibitor) using piroxicam as the internal standard. The mobile phase containing methanol, acetonitrile and an aqueous solution of diammonium hydrogenorthophosphate (50 mM) in the ratio of 4:1:5 was pumped at the rate I ml/min. Lichrocart RP-18 (125×4 mm) was used as an analytical column and the analytes were detected at 364 nm using a UV detector. Acidified plasma samples were extracted with chloroform, evaporated to dryness, reconstituted in the mobile phase and then a volume of 10 μ1 of the prepared sample was injected in the column. The retention time of meloxicam and piroxicam was found to be 2.7 and 1.9, respectively. This method showed an accuracy of 102.3% at 0.52 μg/ml and was capable of detecting a minimum concentration of 0.029 μg/ml meloxicam from biological samples. The analytical method was successfully utilized for estimating meloxicam in biological samples.

Revue / Journal Title

Journal of chromatography. B, Biomedical sciences and applications   ISSN 1387-2273 

Source / Source

2000, vol. 738, n^o2, pp. 431-436 (5 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1997-2001) (Revue)

Mots-clés anglais / English Keywords

Human ; Biological fluid ; Development ; Validation ; Pharmacokinetics ; Meloxicam ; HPLC chromatography ; Extraction ; Estimation ; Analytical method ; Quantitative analysis ; Non steroidal antiinflammatory agent ; Enzyme inhibitor ;

Mots-clés français / French Keywords

Homme ; Liquide biologique ; Développement ; Validation ; Pharmacocinétique ; Méloxicam ; Chromatographie HPLC ; Extraction ; Estimation ; Méthode analytique ; Analyse quantitative ; Antiinflammatoire non stéroïde ; Inhibiteur enzyme ;

Mots-clés espagnols / Spanish Keywords

Hombre ; Líquido biológico ; Desarrollo ; Validación ; Farmacocinética ; Meloxicam ; Cromatografía HPLC ; Extracción ; Estimación ; Método analítico ; Análisis cuantitativo ; Antiinflamatorio no esteroide ; Inhibidor enzima ;

Localisation / Location

INIST-CNRS, Cote INIST : 8577 B, 35400008662364.0260