Titre du document / Document title

Optimization of microbiological parameters for enhanced griseofulvin production using response surface methodology

Auteur(s) / Author(s)

VENKATA DASU V. ⁽¹⁾ ; PANDA T. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Biochemical Engineering Laboratory, Biotechnology Research Centre, Indian Institute of Technology-Madras, Chennai-600 036, INDE

Résumé / Abstract

Central composite design was used to determine the optimal levels of microbiological parameters, viz., slant age, seed age and inoculum level, for enhanced griseofulvin production by Penicillium griseofulvum MTCC 1898 and Penicillium griseofulvum MTCC 2004 in shake flask fermentation. The optimal levels of slant age, seed age and inoculum level for Penicillium griseofulvum MTCC 1898 were found to be 8.8772 days, 4.2093 days, 12% (v/v) (≡17.56 kg dry cell mass/m³) and for Penicillium griseofulvum MTCC 2004, 8.221 days, 3.4875 days and 9% (v/v) (≡8.09 kg dry cell mass/m³) respectively. The yield of griseofulvin under optimal conditions was found to be 1.65 times for Penicillium griseofulvum MTCC 1898 and 1.07 times for Penicillium griseofulvum MTCC 2004 higher than that obtained using unoptimized conditions. The fermentation time for maximum production of griseofulvin by Penicillium griseofulvum MTCC 1898 and Penicillium griseofulvum MTCC 2004 decreased by 4 days and 2 days respectively.

Revue / Journal Title

Bioprocess engineering   ISSN 0178-515X   CODEN BIENEU 

Source / Source

2000, vol. 22, n^o1, pp. 45-49 (16 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Berlin, ALLEMAGNE  (1986-2000) (Revue)

Mots-clés anglais / English Keywords

Fermentation ; Production ; Antibiotic ; Antifungal agent ; Optimization ; Operating conditions ; Culture medium ; Experimental design ; Response surface ; Griseofulvin ; Fungi Imperfecti ; Fungi ; Thallophyta ;

Mots-clés français / French Keywords

Fermentation ; Production ; Antibiotique ; Antifongique ; Optimisation ; Condition opératoire ; Milieu culture ; Plan expérience ; Surface réponse ; Griséofulvine ; Penicillium griseofulvum ; Fungi Imperfecti ; Fungi ; Thallophyta ;

Mots-clés espagnols / Spanish Keywords

Fermentación ; Producción ; Antibiótico ; Antifúngico ; Optimización ; Condición operatoria ; Medio cultivo ; Plan experiencia ; Superficie respuesta ; Griseofulvina ; Fungi Imperfecti ; Fungi ; Thallophyta ;

Localisation / Location

INIST-CNRS, Cote INIST : 21207, 35400008154891.0090