Titre du document / Document title

Effect of diabetes and insulin treatment on nitric oxide synthase content in rat corpus cavernosum

Auteur(s) / Author(s)

XU Zhi-Shun ⁽¹⁾ ; QIANG FU ⁽²⁾ ; ZHAO Sheng-Tian ⁽¹⁾ ; LIU Hai-Nan ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Urology, Qilu Hospital, Shandong University, Jinan 250012, CHINE
⁽²⁾ Department of Urology, Shandong Provincial Hospital, Jinan 250021, CHINE

Résumé / Abstract

Aim: To study the effect of diabetes mellitus and insulin treatment on rat penile nitric oxide synthase content. Methods: Male Wistar rats were divided at random into two groups: the Control (n = 8) and the Diabetic ( n = 17). Diabetes mellitus was induced by intraperitoneal injection of streptozotocin. The diabetic animals were then randomly divided into two subgroups : diabetic rats without insulin treatment (n = 7) and diabetic rats with insulin treatment ( n = 10). The neuronal nitric oxide synthase (nNOS) in the penile corpus cavernosum were assayed by immumohistochemical staining with specific antibody to nNOS and the nNOS-positive nerve fibers were counted semiquantitatively under a high power microscope. Results: The nNOS- positive nerve fibres in diabetic rats with treatment was higher than that in diabetic rats without treatment ( P < 0.05) and lower than that in the controls (P < 0.01) The nNOS-positive nerve fibres in diabetic rat without treatment were also lower than that in the controls (P < 0.01) Conclusion: In streptozotocin-induced diabetic rats, the nNOS content in the penile corpus cavernosum was significantly decreased. Insulin treatment at the dose level employed partially restores the penile nNOS content in these rats.

Revue / Journal Title

Asian journal of andrology   ISSN 1008-682X 

Source / Source

2001, vol. 3, n^o2, pp. 139-142 (17 ref.)

Langue / Language

Anglais

Editeur / Publisher

blackwell, Oxford, ROYAUME-UNI  (1999) (Revue)

Mots-clés anglais / English Keywords

Diabetes mellitus ; Chemotherapy ; Treatment ; Insulin ; Enzymatic activity ; Nitric-oxide synthase ; Corpus cavernosum ; Impotence ; Complication ; Animal model ; Pathophysiology ; Animal ; Rat ; Oxidoreductases ; Enzyme ; Rodentia ; Mammalia ; Vertebrata ; Endocrinopathy ; Male genital diseases ; Erection disorders ;

Mots-clés français / French Keywords

Diabète ; Chimiothérapie ; Traitement ; Insuline ; Activité enzymatique ; Nitric-oxide synthase ; Corps caverneux ; Impuissance ; Complication ; Modèle animal ; Physiopathologie ; Animal ; Rat ; Oxidoreductases ; Enzyme ; Rodentia ; Mammalia ; Vertebrata ; Endocrinopathie ; Appareil génital mâle pathologie ; Erection pathologie ;

Mots-clés espagnols / Spanish Keywords

Diabetes ; Quimioterapia ; Tratamiento ; Insulina ; Actividad enzimática ; Nitric-oxide synthase ; Cuerpo cavernoso ; Impotencia ; Complicación ; Modelo animal ; Fisiopatología ; Animal ; Rata ; Oxidoreductases ; Enzima ; Rodentia ; Mammalia ; Vertebrata ; Endocrinopatía ; Aparato genital macho patología ; Erección patología ;

Localisation / Location

INIST-CNRS, Cote INIST : 26989, 35400009784365.0110