Titre du document / Document title
Optimisation of the second phase of a two phase growth system for anthocyanin accumulation in callus cultures of Rudbeckia hirta
Auteur(s) / Author(s)
LUCZKIEWCZ M. (1) ;
CISOWSKI W. (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Pharmacognosy, Faculty of Pharmacy, Medical University of Gdansk, Al. Gen. J. Hallera 107, 80-416 Gdansk-Wrzeszcz, POLOGNE
Résumé / Abstract
A two-phase growth system composed of modified Schenk-Hildebrandt medium and enriched Miller's medium was used to grow callus of R. hirta L. The second phase of the system has been optimised and the callus synthesised a 12-compound set of anthocyanins, comprising 4.97% of tissue mass (tubular flowers of the plant in nature only contain one compound comprising 0.28%). The beneficial effect of high content of cysteine on anthocyanin accumulation was noted. The predominant element of the anthocyanin set obtained in vitro is cyanidin-3-O-(6-O-malonyl-β-D-glucopyranoside), a relatively stable compound.
Revue / Journal Title
Plant cell, tissue and organ culture
ISSN
0167-6857
CODEN PTCEDJ
Source / Source
2001, vol. 65, n
o1, pp. 57-68 (27 ref.)
Langue / Language
Anglais
Editeur / Publisher
Springer, Heidelberg, ALLEMAGNE
(1981)
(Revue)
Mots-clés anglais / English Keywords
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
Mots-clés français / French Keywords
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
Mots-clés espagnols / Spanish Keywords
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
;
Localisation / Location
INIST-CNRS, Cote INIST : 19374, 35400009556961.0080
Nº notice refdoc (ud4) : 1072932
